Similar results were also observed in the number of cells crossing the transwell chamber (6–10B: 56 ± 6, 6–10B-KO-1: 111 ± 11, 6–10B-KO-2: 125 ± 7, p < 0.05; Tu686: 82 ± 8, Tu686-KO: 123 ± 9, p < 0.05), which implied that PRAS40 might inhibit HNSCC cell metastasis. Here, AKT1S1 is linked to head and neck squamous cell carcinoma.