The results showed that the serum concentrations of six biomarkers were significantly higher in LC patients than in BLD controls: CYFR21 (P = 0.008), CEA (P = 0.004), NSE (P = 0.0041), CA125 (P < 0.0001), CA15-3 (P < 0.0001), and CA19-9 (P = 0.0203). The gene discussed is CEACAM5; the disease is laryngotracheoesophageal cleft.