The variables tested by univariate analysis were sex, age, hepatitis history, factors of primary curative resection, and features before RFA including total bilirubin (TB), alanine aminotransferase (ALT), prothrombin time (PT), albumin, γ-glutamyltranspeptidase (GGT), AFP, tumor size, and tumor number. This evidence concerns the gene F2 and hepatitis A virus infection.