The variables tested by univariate analysis were sex, age, hepatitis history, factors of primary curative resection, and features before RFA including total bilirubin (TB), alanine aminotransferase (ALT), prothrombin time (PT), albumin, γ-glutamyltranspeptidase (GGT), AFP, tumor size, and tumor number. The gene discussed is AFP; the disease is hepatitis A virus infection.