found the melanoma patient respond to anti-PD1 treatment has higher microbial diversity, including the abundance of Ruminococcaceae, Clostridiales, and Faecalibacterium, patients with more Faecalibacterium has a significantly prolonged progression-free survival with a higher level of effector T cells and a stabilized cytokine response to PD-1 blockade, simultaneously, systemic and anti-tumor immunity are also enhanced (144). The gene discussed is PDCD1; the disease is neoplasm.