No loss of membrane integrity or increase in caspase 3/7 activity could be detected using an LDH and CaspGlo® assay respectively, which indicates the absence of cell lysis or apoptotic cell death of human HCC cells upon treatment with GKT771 at concentrations up to 100 μM (Fig. 2b-c & e-f). Here, CASP3 is linked to hepatocellular carcinoma.