We first examined the expression level of HDAC1 in suspension-cultured spheres (model of CRC-SCs) and adherent cells (model of nsTCs) as well as re-adherent cells (model of differentiated cancer cells) derived from both HCT-116 and HT-29 cell lines and primary cultured CRC cells. The gene discussed is HDAC1; the disease is colorectal carcinoma.