RAB10 and osteosarcoma: Further RT-qPCR and western blot analysis validated that the overexpression of miR-224-5p significantly reduced the expression of Rab10, whereas knockdown of miR-224-5p significantly enhanced the expression of Rab10 at both the mRNA and protein levels in 143B osteosarcoma cells (Fig. 2F,G and Supplementary file).