The fact that ISH for EBER1 is used commonly as a diagnostic test for EBV infection suggests that EBER1 is expressed at levels sufficient to stimulate the TLR or RIG-I pathways in EBV-infected cells, but expression status of others which could stimulate IRF3 expression were unknown in EBV (+) GC. This evidence concerns the gene RIGI and Epstein-Barr virus infection.