TP53 and neoplasm: Thus, by demonstrating positive p53 staining in tumour cells between day 0 and day 12 PDAC explants, and consistent with previous findings in ex vivo cultured PDAC slices26, our results suggest that tumour specific drivers such as p53 (which is mutated in approximately 75% of PDAC patients40,41) are maintained in our PDAC explant model.