CAR-T cells generated using anti-B7H3 scFv fused to 4-1BB and CD3z (Supplementary Fig. 1b) showed significant in vitro proliferation, cytokine production, and specific tumor lysis in the presence of B7H3+ but not B7H3- cells (Fig. 3b–f and Supplementary Fig. 4a–d). This evidence concerns the gene CD276 and neoplasm.