To determine whether macrophages can be directly differentiated into LECs in renal fibrosis, immunofluorescence double-labeling of macrophages (F4/80) and lymphangiogenesis markers (LYVE-1) was conducted to analyze colocalization in UUO (Fig. 3A) and ADR (Fig. 3B) mice, and the results showed that some LYVE-1+ lymphatic vessels were also positive for F4/80. Here, LYVE1 is linked to renal fibrosis.