Tim-3, LAG3, BTLA and CD160 were expressed by a mean of <2% of tumor-infiltrating CD8 T cells, and while CD244 (2B4) was present on higher numbers of CD8 T cells in cSCC, the frequencies of CD244+CD8 T cells did not significantly differ between cSCC, NS and blood (figure 5D). This evidence concerns the gene BTLA and neoplasm.