Of the 136 CRC cases with a DNA methylation signal (i.e. at least 1 PCR replicate positive for methylation in either BCAT1, IKZF1 and/or IRF4), 35 (25.7%) cases were methylation positive in all 9 PCR replicates and 26 (19.1%) were methylation positive by just a single PCR replicate. The gene discussed is IKZF1; the disease is colorectal carcinoma.