BCAT1 and cancer: A high replicate count was characteristic of patients with CRC (a quarter of cancer cases were positive in all nine replicates) while in the majority of cases without neoplasia, only one replicate was positive, usually BCAT1. Based on the cohort tested herein, the odds ratio of a single BCAT1 positive PCR replicate being a false-positive compared to a true positive was 17.7.