(GLUT + ADP) treatment increased NADPH level by 25% in CF cells, with respect to untreated CF cells, and it was strongly sensitive to GSNO which caused a strong reduction of NADPH content (Figure 2A), this confirming that NNT operated in the forward direction, that is it had produced NADPH by likely utilizing NADH, provided by the GLUT + ADP couple, and mΔΨ. Here, SLC2A1 is linked to cystic fibrosis.