One should also be aware of numerous limitations of RDTs (poor sensitivity at low parasite densities, susceptibility to the prozone effect /Pf HRP2-detecting RDTs/), the possibility of false negative results (due to Pf HRP2 deficiency in the case of pfhrp2 gene deletions /Pf HRP2-detecting RDTs/ and cross-reactions between Plasmodium antigens and detection antibodies), as well as the possibility of false-positive results caused by other infections [22]. This evidence concerns the gene HDGFL2 and infection.