CDK4 and hepatocellular carcinoma: Consistent with these data, results of our western blot analysis show that silencing SAE1, elicited upregulated expression of drivers of cancer progression CDK4 and cyclin B1 (a CCNB1 gene product), concomitantly with downregulated tumor suppressors FOXO1 and KLF9 proteins in HCC cell line Huh7 (Figure 5E).