PRF1 and neoplasm: The expression of T cell cytotoxicity (CD3G, CD3E, CD4, GZMA, PRF1 and TBX21), B cell MHC II and immune exhausted-related genes were abundant in metastatic sites; while MHC I inflammation related genes (HLA-B, HLA-C and IL-1A) were mostly higher in primary than recurrent tumor sites (Fig 3A).