RUNX1 and osteoporosis: However, AAV mediated Runx1 overexpression can rescue bone loss in OVX-induced osteoporosis through enhancing osteoblast proliferation and differentiation, which was further enhanced by co-overexpression with Cbfβ. Runx1 overexpression in Runx2-/- newborn mice calvarial cells induced by osteogenesis medium rescued ALP staining, indicating that Runx1 can compensate for loss of Runx2 expression.