In order to assess the impact of the entire manipulation procedure and rule out selection of CD4 T cells with limited fitness, we evaluated engraftment and survival of the edited cells in vivo, by performing adoptive transfer of male bulk edited T cells, NGFR+ enriched T cells or untreated cells into non‐obese diabetic (NOD)‐severe combined immunodeficiency (SCID)‐IL2Rg−/− (NSG) mice. Here, CD4 is linked to severe combined immunodeficiency.