To determine the mechanism of FoxO1 on ROS production in in vitro model of DN, over‐expression of FoxO1 reduced MDA levels and ROS production levels, and enhanced the levels of SOD, GSH, and GSH‐PX in in vitro model of DN, compared to the in vitro model of DN group (Figure 2a‐f). The gene discussed is FOXO1; the disease is liver dysplastic nodule.