We next focused on the core transcription factors involved in myeloid differentiation and found that Runt-related transcription factor 1 (RUNX1), a core-binding factor family member, was significant upregulated upon PKM2 depletion, and was inhibited by re-expression of WT PKM2 but not K270R mutant (KR) in both leukemia cells, indicating that RUNX1 is a molecule highly relevant to PKM2 SUMOylation (Fig. 5B and Supplemental Table 2). Here, PKM is linked to leukemia.