Furthermore, to examine the direct functional effect of N2-neutrophil on lung metastasis in vivo, we performed adoptive transfer of N2-neutrophils (CD11b+Ly6G+) isolated from bone marrow of naive, non-tumor-bearing Balb/c mice treated with nicotine for 10 days (Fig. 3D; Supplementary Fig. 7A, B). Here, ITGAM is linked to neoplasm.