To investigate whether Notch signaling plays a role in regulating MEC, we first determined the Notch signaling status in four human CRTC1-MAML2 fusion-positive MEC cell lines, including H292 (metastatic pulmonary MEC), H3118 (metastatic parotid MEC), UM-HMC-3A and UM-HMC-3B (from a local recurrence and metastatic lymph node from the same patient with MEC, respectively), and a fusion-negative cell line UM-HPA-1 (benign pleomorphic adenocarcinoma). Here, CRTC1 is linked to mucoepidermoid carcinoma.