Taken together, our results demonstrated that combination of vaccination followed by RT significantly induced the systemically high production of IFN-γ in CD8+ T cells; the enhanced MHC-1 expression on the surface of tumor cells and lymphocytes; and the increased chemokine production in the TME that attracted more effector CD8+ T-cell infiltration into the tumor milieu, thus leading to a favorable immune microenvironment. The gene discussed is IFNG; the disease is neoplasm.