The results showed that, after mice received two doses of vaccine and one irradiation, E7 + RT treatment induced much stronger immune response in tumor than that of RT + E7 treatment, characterized by significant improvements in the percentages of CD45+ cells, CD8+ T cells in CD45+ cells, CD8+IFN-γ+ cells, and the ratio of CD8+ T cells to Tregs (Supplementary Fig. 4). Here, IFNG is linked to neoplasm.