The organotypic slices from one liver cancer patient-derived tumor were incubated with peripheral blood mononuclear cells (PBMCs) from the same patient in the presence of Met@Man-MPs derived from THP-1-originated macrophages, anti-PD-1 antibody or both Met@Man-MPs and anti-PD-1 antibody. The gene discussed is PDCD1; the disease is liver cancer.