SLC12A2 and Hydrocephalus: We first overexpressed ChP NKCC1 at E14.5 by in utero AAV2/5 ICV, then introduced obstructive hydrocephalus by a single unilateral injection of kaolin into the lateral ventricle at P455, and finally evaluated the lateral ventricle volumes by live T2 MRI at P14 (Fig. 6a).