We first overexpressed ChP NKCC1 at E14.5 by in utero AAV2/5 ICV, then introduced obstructive hydrocephalus by a single unilateral injection of kaolin into the lateral ventricle at P455, and finally evaluated the lateral ventricle volumes by live T2 MRI at P14 (Fig. 6a). Here, SLC12A2 is linked to obstructive hydrocephalus.