In all three sarcomacell lines (HT1080 fibrosarcoma cells, SK-LMS-1 leiomyosarcoma cells, andDDLS8817 dedifferentiated liposarcoma cells), growth as spheroids increasedexpression of Nanog compared with that in monolayer-grown cells, whileexpression of other self-renewal proteins Sox2, Oct4, and c-Myc was notuniformly increased (Fig. 1A). Here, POU5F1 is linked to leiomyosarcoma.