Furthermore, AHA1 knockdown significantly inhibited both intracellular glutathione (GSH) biosynthesis and ATP consumption, while AHA1-overexpressed cells showed increased intracellular glutathione biosynthesis and ATP consumption (Fig. 4b, c, e, f), indicating AHA1 positively regulates biomass biosynthesis and energy consumption in osteosarcoma cells. Here, AHSA1 is linked to osteosarcoma.