To meet the huge demand for energy and biomass generation, tumor cells tend to enhance their metabolic activity through upregulating the level and activity of enzymes that are critical for cellular metabolism.26,27 In this study, we demonstrated that AHA1 facilitates the metabolic activity of osteosarcoma cells, including the consumption of glucose and ATP, production of lactate and glutathione, which is associated with glycolysis and TCA cycle. The gene discussed is AHSA1; the disease is osteosarcoma.