Interestingly, EVs isolated from irradiated tumour cells (H22 cells and 4T1 cells; 8 Gy) in vitro were shown to have immunomodulatory effects when mice were inoculated in vivo, enhancing CD8+ and CD4+ T-cell infiltration in lung metastasis in comparison to nonirradiated EVs [25]. This evidence concerns the gene CD8A and neoplasm.