To further investigate the pathology of AMS mice at the molecular and cellular levels, we generated three different NSC lines from embryonic brains of wild-type, Nna1 mutation heterozygous, and AMS (Nna1 mutation homozygous) mice, named NMW7, NME, and NMO1, respectively. This evidence concerns the gene OPN3 and ablepharon macrostomia syndrome.