To compare the exosome formation in colon cancer cell lines expressing different mutation statuses, three colon cell lines, HT29 (wild type KRAS), SW480 (KRAS G12V mutation) and HCT116 (KRAS G13D mutation), were transfected with plasmid pCT-CD63-GFP. The gene discussed is CD63; the disease is malignant colon neoplasm.