TNF and melanoma: We found that PD-1 and PD-L1 monotherapies, as well as their combination with TIM-3 blockade, were associated with the highest secretion of immune related-cytokines and granzyme B. Increased gene expression or cytokine secretion levels for IFNg, TNFa, IL-10, and granzyme B in response to IC therapy have also been observed in vivo in AB1-HA and AE17 mesothelioma mouse models [19], as well as in other murine tumor models, such as B16 melanoma and MC38 colon carcinoma [20,21].