Therefore, we harvested tumors and spleens from BALB/cJ mice before the first treatment and we looked at the expression of PD-1, PD-L1, TIM-3, and LAG-3 on CD4 and CD8 effector lymphocytes in the spleen as well as on tumor infiltrating lymphocytes (TILs). The gene discussed is CD274; the disease is neoplasm.