Numerous in vitro studies, using different expression models and a number of functional assays, have been performed for uncovering the disease-associated mechanisms of several dozen Cx26 mutants potentially involved in hearing loss [10,24,25,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48]. Here, GJB2 is linked to hearing loss disorder.