Paxillin antisense RNA 1 (PXN-AS1), a lncRNA overlapping PXN mRNA, was identified after discovering alternative splicing events on a transcriptome sequencing analysis of a hepatocellular carcinoma (HCC) cell line with stable deletion of Muscleblind-like-3 (MBNL3), an oncofetal splicing factor. Here, PXN is linked to hepatocellular carcinoma.