To investigate the functional role of TWIST1 in OSCC, we studied this gene in a syngeneic model using the murine oral carcinoma cell line (AT84), which expresses high levels of TWIST1 and also a highly metastatic human cell line (OSCC1.2/RBT3) using nude mice (Figure 6A). Here, TWIST1 is linked to lip and oral cavity carcinoma.