The tumor samples were stained for CD68 (total macrophage marker), CD163, CD206, CD204, CHID1 (M2 markers), inducible nitric oxide synthase (iNOS), IDO1 (M1 marker), FoxP3 (Treg marker), CD3 (mature T-cells marker), CD8, and PD-L1. Here, NOS2 is linked to neoplasm.