To determine whether PP55L amino acid substitution affects the IFN response in cells, HINAE cells were infected with 1 MOI of rVHSV-wild or rVHSV-P, total RNA was extracted at 6, 12, and 24 h post-infection, and the induction of IFN1 and Mx gene expression was analyzed by real-time PCR. Here, IFNA1 is linked to infection.