To test this possibility in mice as a model, we generated mouse melanoma cell line B16 expressing membrane-anchored from of hen egg lysozyme (mHEL) [14] as a surrogate tumor antigen, and generated iGB cells from spleen B cells of Hy10 mice, which carry a hen egg lysozyme (HEL)-specific heavy chain (VDJ9) and light chain (κ5) genes in knock-in and transgenic configurations, respectively [15]. This evidence concerns the gene LYZ and melanoma.