Nevertheless, the expression of other genes involved in IMCL turnover, PLIN2 (perilipin 2), and lipid intermediate metabolism, DGKD (diacylglycerol kinase delta), was increased with carnitine supplementation, and it is generally accepted that the accumulation of metabolites associated with IMCL (i.e. acyl‐CoA, diacylglycerol and ceramide) is a major cause of insulin resistance in skeletal muscle with age (Lee et al., 2010; Savage et al., 2007). This evidence concerns the gene DGKD and Insulin resistance.