Based on the pseudo-temporal continuum profile, we identified the TF (ALX4, HINFP, CEBPA, CEBPB, DMBX1, MLXIPL, ONECUT1, and RBPJL) and depicted the regulated kernel genes co-networks (Fig. 4D), which were subjected to the metabolism disorders (Fig. 4E). This evidence concerns the gene ALX4 and Other metabolic disease.