PD-L1 expression, as assessed using IHC, failed to predict the efficacy of ICIs in CRC.35 Data from a number of recent studies have proposed that the level of PD-L1 mRNA strongly correlated with prognosis and immune infiltration of tumors, especially in hepatocellular carcinoma (HCC), NSCLC, and malignant melanoma.12–14 To date, no study has investigated this predictive value of PD-L1 mRNA in CRC. This evidence concerns the gene CD274 and non-small cell lung carcinoma.