CD274 and malignant colon neoplasm: To further validate the role of miR-15b-5p in PD-L1-mediated immunosuppression, we infected mice colons with AAV carrying an antisense sequence (miR-15b-5p sponge vector) to inhibit the miR-15b-5p expression and evaluated the effects of miR-15b-5p on tumorigenesis in CAC and APCmin/+ colon cancer murine models (figure 3A, B).