Inspired by previous report that chemotherapeutic drugs could promote the secretion of extracellular vesicles carrying the multidrug resistance transporter ABCB1 from multidrug resistant cancer cells and transfer to neighboring sensitive cells [31], the effect of osimertinib on exosomes biogenesis was also investigated in wtEGFR-expressing NSCLC cells in our study. The gene discussed is ABCB1; the disease is cancer.