To ascertain whether the intercellular transfer of exosomal wtEGFR protein activates EGFR downstream signaling pathway in mutEGFR cancer cells under osimertinib treatment, the status of the PI3K/AKT and MAPK pathways was evaluated after incubation with the CM from wtEGFR-bearing NSCLC cells. This evidence concerns the gene AKT1 and non-small cell lung carcinoma.