To sum up, we surmised that the action mechanism of polypharmacology molecules of Epimedium in treating NSCLC might be via targeting TNF, PI3K-Akt and VEGF signaling pathways in the TME to regulate multiple targets in multiple cells and exert effects of anti-inflammatory, promote apoptosis and anti-migration. This evidence concerns the gene TNF and non-small cell lung carcinoma.