The mice were also fed a low-fat AIN76 diet containing 4.2% fat and 0.02% cholesterol, which has been successfully used in many studies to induce atherosclerosis in LDLR−/− or ApoE−/− mice without eliciting obesity and associated metabolic disorders [43, 60, 45]. The gene discussed is LDLR; the disease is obesity due to melanocortin 4 receptor deficiency.