CD8A and infection: However, a more detailed analysis of memory CD8+ T cell differentiation by Cytosplore (Höllt et al., 2016), which incorporates approximated t-distributed stochastic neighborhood embedding (A-tSNE) algorithms for subset definition, revealed a difference in the heterogeneity of the GP33-specific CD8+ T cells in blood at both the acute and memory phase of infection (Figures 1C, S1D, and S1E).