The CP or cycle threshold was calculated from the amplification plot of the individual reaction graph post the application of 0.07 thresholds. The geometric mean for GAPDH, RPNI, PUM1, B2M, and PMM1 were reported as 15.55, 19.73, 12.22, 18.44, and 21.87 in rectal tumor samples, respectively. This evidence concerns the gene B2M and rectal neoplasm.