RASSF1 and cystic fibrosis: Consistent with the results of bisulfite sequencing, the results of Western blotting revealed that the protein expressions of Rasal1 and Rassf1 were markedly depressed in CF hearts (P < 0.05), and DHI treatment significantly upregulated protein expressions of Rasal1 (P < 0.05) and Rassf1 (P < 0.01) (Figures 7(a) and 7(b)).