However, focusing on the methylation balancing effect of DHI in vivo, we did not investigate the direct relationship between DNMT3B and Ras-signal gene hypermethylation in vitro assays, and further exploration is required to elucidate the benefits of DHI as a complementary therapy for diastolic heart failure and its methylation effect. The gene discussed is DNMT3B; the disease is diastolic heart failure.