Given the deficit of reliable biomarkers for NSCLC CSCs, we obtained potential CSC subpopulations from NSCLC cell lines based on general traits of CSCs, including tumor sphere-forming capacities 45, expression of CSC markers, such as Oct4, Nanog, and Sox2 46, and ALDH activity 47. This evidence concerns the gene POU5F1 and non-small cell lung carcinoma.