Having previously shown the role of Nanog and Oct4 in the regulation of CSCs derived from lung cancer 7, we next selected putative NSCLC CSCs by establishing H460 and H1299 NSCLC cells stably transfected with green fluorescent protein (GFP) reporter vectors carrying human NANOG or POUSF1 promoters (H1299/pOct4-GFP, H1299/pNanog-GFP, H460/pOct4-GFP, and H460/pNanog-GFP). Here, POU5F1 is linked to lung cancer.