In this study, the effect of the FGFR4 p.Gly388Arg variant on 49 nonmutated patients with retinoblastoma of the RB1 gene, their 13 healthy relatives and 146 age- and gender-matched control groups, and the FGFR4 p Gly388Arg variant on patients with retinoblastoma and control groups used the bidirectional sequencing Sanger Sequencing process. This evidence concerns the gene RB1 and retinoblastoma.