Based on the important role of APN in regulating fatty acid oxidation and improving insulin resistance and the vertical effect of PCOS maternal endocrine environment on female offspring, this study focused on female offspring of obese mice with PCOS by observing the relevant metabolic phenotypes of each offspring after reaching adulthood to analyze the effect of APN supplementation during early embryo development on PCOS-related metabolic disorders in adult female offspring and further explore its mechanism. This evidence concerns the gene ANPEP and Insulin resistance.